Mitochondrial DNA was extracted from the crime scene evidence in 1999/2000, leading to a DNA concentration of 0.003 nanograms per microliter being retrieved from a discarded cigarette butt found in the alleyway, and 0.01 nanograms per microliter being retrieved from the hair discovered at the base of Cheri Jo Bates' right thumb. There is however, no mention of the blood from the alleyway floor. This blood trail could be a combination of blood from the knife and blood from the killer, but the longer the trail extends, the likelier it becomes that it originates from the bloodstream of the murderer.
Assuming that the blood was retrieved along the whole trail to Terracina Drive, it would have been extremely routine forensic work in 1966 to determine the blood group of the submitted samples. Cheri Jo Bates' blood type detailed at autopsy is rare in the USA population. It was AB RhD positive, which accounts for only 3.4% of the American population. Any samples submitted that were not an AB blood group were almost certainly from her killer. However, even if the submitted samples were of the same blood group as Cheri Jo Bates, it still doesn't eliminate the possibility of the samples having originated from her murderer. He may have had the same blood type.
The tail of the blood drops would have indicated the directional movement of the killer towards Terracina Drive (as opposed to away), but should also have revealed which side of the alleyway the killer was favoring as he headed towards Terracina Drive - and the likely location of his parked vehicle (assuming he had one). If he had no vehicle, then it could have told us which side of the city he was heading to reach his residence. The diameter of the blood drops (reducing in size or not) could also tell us whether this was a freshly dripping wound, or the gradually reducing volume of blood falling from a knife blade. If the killer was telling the truth when he stated "I plunged the knife into her and it broke", then it is quite possible there was no knife to be dripping. No broken blade was found at autopsy, so it is perfectly conceivable that the hinge mechanism of the small pocket knife may have given way when striking the back of Cheri Jo Bates.
A female earwitness described "an awful scream between 10:15 pm and 10:45 pm, and then about two minutes of silence, and finally the sound of an old car starting up". If the author of the Confession Letter was really the killer of Cheri Jo Bates, then we can estimate the location of his vehicle, assuming the earwithness was hearing the actual murder take place. By combining the direction of the blood trail to one side of Terracina Drive, with the two minute interval from "scream to vehicle starting up", we can use the details in the Confession Letter to pinpoint fairly accurately the location of his vehicle.
The Confession Letter stated "She let out a scream once and I kicked her in the head to shut her up. I plunged the knife into her and it broke. I then finished the job out cutting her throat". If this is when he "finished the job out" before leaving the scene, then he had two minutes to "kick her in the head, plunge the knife into her, cut her throat" and walk to his waiting vehicle. Allotting about 45 seconds at the murder scene, he has approximately 75 seconds to negotiate the 100 feet to Terracina Drive, and the rest to reach his vehicle. Traveling at an average walking speed of 1.4 meters per second, the 100 feet (30.48 meter) journey would take about 22 seconds, leaving 53 seconds walking time on Terracina Drive. That would mean a vehicle parked 243 feet (74 meters or 81 yards) from the alleyway. Cheri Jo Bates' Volkswagen Beetle was parked 75 yards (68 meters) east of the alleyway, just beyond the library annex. This rough estimate, would place the killer's vehicle only 6 yards (18 feet) behind the vehicle of Cheri Jo Bates (had he exited the alleyway east) - the ideal spot to offer her assistance when her vehicle failed to start - and the near exact location of the Tucker Torpedo (or Studebaker) described in Robert Graysmith's book.